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- Screening
- Fragment Screen
- XP Screens
- Crystal Screens
- Thermofluor Screens
- Detergent Screens
- Additive Screens
- Buffer Screens
- Solubility & Stability Screens
- Plates & Accessories
- Oils & Dyes
- Screening Membrane Proteins
- Optimization
- Data Collection
- Phasing
- Cryo-EM
Detergent Screens
JBScreen Detergents contain 4 x 24 unique detergents that are compatible with most common crystallization reagents and are therefore perfectly suited for membrane protein solubilization:
- Ionic detergents
- Non-ionic detergents
- Zwitterionic detergents
- Non-detergent Sulfobetaines
- Synthetic Lipids
JBScreen Detergents can be used throughout the protein purification process or can be added afterwards by dialysis or ion-exchange chromatography (detergent exchange). Detergent exchange can be vital for obtaining well-diffracting membrane-protein crystals [1].
JBScreen Detergents is also valuable for additive screening with detergents and detergent mixtures [2,3] in combination with the JBScreen Membrane. This combination will enable you to screen a broad range of detergents, while concentrating on the most successful crystallization conditions, making crystallization screening of membrane proteins much more efficient and less time consuming.
JBScreen Detergents & Prometheus: A winning team
Screen for stabilizing detergents by combining JBScreen Detergents with Prometheus NT.48, a label-free nanoDSF technology developed by NanoTemper Technologies, ideally suited for membrane protein stabilization.
Products & Ordering
References
[1] Rosenow et al. (2003) The influence of detergents and amphiphiles on the solubility of the light harvesting complex. Acta Cryst. D59:1422
[2] Adir (1999) Crystallization of the oxygen-evolving reaction centre of photosystem II in nine different detergent mixtures. Acta Cryst. D55:891
[3] Koronakis et al. (2000) Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export. Nature 405:914
Selected Literature Citation of JBScreen Detergents
- Hofmann et al. (2020) High-Level Expression, Purification and Initial Characterization of Recombinant Arabidopsis Histidine Kinase AHK1. Plants 9(3):304.
- Delle Bovi et al. (2017) Expression and purification of functional insulin and insulin-like growth factor 1 holoreceptors from mammalian cells. Anal. Biochem. DOI 10.1016/j.ab.2017.08.011.
- Hofmann et al. (2020) High-Level Expression, Purification and Initial Characterization of Recombinant Arabidopsis Histidine Kinase AHK1. Plants 9(3):304.
- Delle Bovi et al. (2017) Expression and purification of functional insulin and insulin-like growth factor 1 holoreceptors from mammalian cells. Anal. Biochem. DOI 10.1016/j.ab.2017.08.011.