Wako和光纯药Insect Cell Culture Medium: WakoVAC PSFM-J1

Insect Cell Culture Medium: WakoVAC PSFM-J1

Licensed Formulation

Formulated for insect cell expansion. Designed to maximize insect cell growth, viability and productivity. WakoVAC PSFM-J1 manufactured in Wako outperforms the same formulation manufactured in competitor’s site due to using quality raw materials and strict and robust raw material control.

Raw Material

All raw materials are documented and traceable. All raw materials used in WakoVAC PSFM-J1 are standardized to a certain specification, scalable, and provided from validated suppliers. WakoVAC PSFM-J1 contains fish-derived component (cGMP, virus-inactivated) and Hydorlysate. Without fish-derived component, the media can be defined animal component-free.

Industrial Applications

The intended use of WakoVAC (GMP-compliant manufactured) is for research, process development, large-scale manufacturing/commercialization. WakoVAC is scalable to commercial volumes for industrial application. Both liquid and powder are available. Flexible packaging is also available upon request. Wako provides complete workflow support from media optimization service to regulatory support at the time of IND/NDA.

Performance

WakoVAC PSJF-J1 supports a wide range of insect cells: Sf+, Hi5, Sf9, Sf21. It is designed to maximize and consistent insect cell growth, viability and sustains high productivity. Best in class performance, Easy to use, Ongoing stability.

  • Brochure

  • Features
  • Culturing Sf9 Cells
  • Culturing Hi5 Cells
  • Target expression data with Sf21
  • Product List
  • Related Product List
  • Related Information

Features

  • Support wide range of insect cells
  • Performs best with Sf+
    • Cell Growth

      Insect Cell Culture Medium: WakoVAC PSFM-J1

    • Cell Viability

      Insect Cell Culture Medium: WakoVAC PSFM-J1

    Insect Cell Culture Medium: WakoVAC PSFM-J1

  • Fish-derived component(cGMP) and hydrolysate contained
  • CoA for non-GMP and GMP available
    Cell growth, cell viability, and protein expression data can be included on CoA upon request
  • Intended use: for research and further manufacturing
Insect Cell Culture Medium: WakoVAC PSFM-J1
Wako Cat# 160-25851 TBD
Format Liquid Powder
Package 1L 8kg and/or Customs upon request
Shelf-life 1 year from the date of MFG 2 years from the date of MFG
Storage Condition 2-10˚C, protect from light

Other Information

  • Assure lot-to-lot consistency
  • Fine protein expression as well with Sf9, Sf21 and Hi5
  • L-Glutamine does not need to be supplemented
  • Comparative Landscape

    Product Description Supplier SF ACF PF CD GMP Hydrolysate Cell Type Shelf-life Storage Size Bag Avaiability
    Sf Insect FUJIFILM Irvine Scientific Yes Yes     Yes + Sf9, Sf21 1 year 2-8℃ 1L 5L(MTO) Custom upon request
    WakoVAC PSFM-J1 FUJIFILM Wako Yes Fish-derived component Yes   Yes ++ Sf+, Hi5, Sf9, and Sf21 1 year 2-10℃ 1L 10L and 20L Powder available

Culturing Sf9 Cells

Retain robust cell growth, viability and target expression with Sf9

Cell Growth
Insect Cell Culture Medium: WakoVAC PSFM-J1
Protein Expression Testing
Insect Cell Culture Medium: WakoVAC PSFM-J1
M: Molecular weight size marker
Lane 1: Wako VAC PSFM-J1
Lane 2: Medium B Control
: Target protein
  • The amount of samples being used: 20μl/Lane
  • SDS-PAGE: SuperSep™ Ace 10-20% (Wako Code: 191-15031),
    200v constant voltage for 60 minutes
  • Western blotting: PVDF membrane
  • Antibody: Anti- his Tag antibody

* Data provided by the Goshima Lab from Nagoya University

Cell Viability
Insect Cell Culture Medium: WakoVAC PSFM-J1

Culturing Hi5 Cells

Retain robust cell growth, viability and target expression with Hi5

Cell Growth
Insect Cell Culture Medium: WakoVAC PSFM-J1
Protein Expression Testing
Insect Cell Culture Medium: WakoVAC PSFM-J1
M: Molecular weight size marker
Lane 1: Wako VAC PSFM-J1
Lane 2: Wako VAC PSFM-J1 + L-Glutamine
Lane 3: Medium C Control + L-Glutamine
: Target protein
  • The amount of samples being used: 20μl/Lane
  • SDS-PAGE: SuperSep™ Ace 10-20% (Wako Code: 191-15031),
    200v constant voltage for 60 minutes
  • Western blotting: PVDF membrane
  • Antibody: Anti- his Tag antibody

* Data provided by Mirai-Kougaku

Cell Viability
Insect Cell Culture Medium: WakoVAC PSFM-J1

Target expression data with Sf21

Retain target expression with Sf21

Protein Expression Testing

Insect Cell Culture Medium: WakoVAC PSFM-J1

M: Molecular weight size marker
Lane 1: Medium A (Pre-viral inoculation)
Lane 2: Medium A (Post-viral inoculation)
Lane 3: PSFM-J1 (Pre-viral inoculation)
Lane 4: PSFM-J1 (Post-viral inoculation)
: Target protein
  • The amount of samples being used: 15μl/Lane
  • SDS-PAGE: 5-20% gradient gel, 200v constant
  • Voltage for 50 minutes

* Data provided by the Goshima Lab from Nagoya University

Product List

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Polyacrylamide gel SuperSep™ Ace

Related Information

Category

  1. Cell Culture
  2. Insect Cell Culture
  3. Medium
  1. Cell Culture
  2. Vaccine Manufacturing
  3. Medium

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