Insect Cell Culture Medium: WakoVAC PSFM-J1
Licensed Formulation
Formulated for insect cell expansion. Designed to maximize insect cell growth, viability and productivity. WakoVAC PSFM-J1 manufactured in Wako outperforms the same formulation manufactured in competitor’s site due to using quality raw materials and strict and robust raw material control.
Raw Material
All raw materials are documented and traceable. All raw materials used in WakoVAC PSFM-J1 are standardized to a certain specification, scalable, and provided from validated suppliers. WakoVAC PSFM-J1 contains fish-derived component (cGMP, virus-inactivated) and Hydorlysate. Without fish-derived component, the media can be defined animal component-free.
Industrial Applications
The intended use of WakoVAC (GMP-compliant manufactured) is for research, process development, large-scale manufacturing/commercialization. WakoVAC is scalable to commercial volumes for industrial application. Both liquid and powder are available. Flexible packaging is also available upon request. Wako provides complete workflow support from media optimization service to regulatory support at the time of IND/NDA.
Performance
WakoVAC PSJF-J1 supports a wide range of insect cells: Sf+, Hi5, Sf9, Sf21. It is designed to maximize and consistent insect cell growth, viability and sustains high productivity. Best in class performance, Easy to use, Ongoing stability.
- Brochure
- Features
- Culturing Sf9 Cells
- Culturing Hi5 Cells
- Target expression data with Sf21
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- Related Information
Features
- Support wide range of insect cells
- Performs best with Sf+
- Cell Growth
- Cell Viability
- Cell Growth
- Fish-derived component(cGMP) and hydrolysate contained
- CoA for non-GMP and GMP available
Cell growth, cell viability, and protein expression data can be included on CoA upon request - Intended use: for research and further manufacturing
| Wako Cat# 160-25851 | TBD | |
|---|---|---|
| Format | Liquid | Powder |
| Package | 1L | 8kg and/or Customs upon request |
| Shelf-life | 1 year from the date of MFG | 2 years from the date of MFG |
| Storage Condition | 2-10˚C, protect from light | |
Other Information
- Assure lot-to-lot consistency
- Fine protein expression as well with Sf9, Sf21 and Hi5
- L-Glutamine does not need to be supplemented
- Comparative Landscape
Product Description Supplier SF ACF PF CD GMP Hydrolysate Cell Type Shelf-life Storage Size Bag Avaiability Sf Insect FUJIFILM Irvine Scientific Yes Yes Yes + Sf9, Sf21 1 year 2-8℃ 1L 5L(MTO) Custom upon request WakoVAC PSFM-J1 FUJIFILM Wako Yes Fish-derived component Yes Yes ++ Sf+, Hi5, Sf9, and Sf21 1 year 2-10℃ 1L 10L and 20L Powder available
Culturing Sf9 Cells
Retain robust cell growth, viability and target expression with Sf9
Cell Growth |
Protein Expression Testing
* Data provided by the Goshima Lab from Nagoya University |
|
Cell Viability |
Culturing Hi5 Cells
Retain robust cell growth, viability and target expression with Hi5
Cell Growth |
Protein Expression Testing
* Data provided by Mirai-Kougaku |
|
Cell Viability |
Target expression data with Sf21
Retain target expression with Sf21
Protein Expression Testing
| M: Molecular weight size marker Lane 1: Medium A (Pre-viral inoculation) Lane 2: Medium A (Post-viral inoculation) Lane 3: PSFM-J1 (Pre-viral inoculation) Lane 4: PSFM-J1 (Post-viral inoculation) ←: Target protein |
- The amount of samples being used: 15μl/Lane
- SDS-PAGE: 5-20% gradient gel, 200v constant
- Voltage for 50 minutes
* Data provided by the Goshima Lab from Nagoya University
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Polyacrylamide gel SuperSep™ Ace
Related Information
Category
- Cell Culture
- Insect Cell Culture
- Medium
- Cell Culture
- Vaccine Manufacturing
- Medium
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