Neuron Dissociation Solutions
Our neuron dissociation solutions can be used to dissociate and isolate neurons from tissues derived from the central nervous system of rat and mouse. It is composed of 3 solutions (enzyme, dissociation, and isolation solutions).
The product is ready-to-use without the need for preparations, and achieves simple isolation of neurons while maintaining high cell viability.
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- Protocol
- Dissociation and Isolation of Frozen Neurons
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Features
- Simple and stable isolation of neurons
- Ready to Use
Protocol
Reagents needed
- Frozen neuron series
- Neuron dissociation solutions (code No. 291-78001) or neuron dissociation solutions S (code No. 297-78101)
- Neuron culture medium (code No. 148-09671)
When using frozen neurons
4. Washing solution: Leibovitz’s L-15 medium (code No. 128-06075) or HPSS (+) (code No.128-06075)
Experimental protocol
Recommended volumes of dissociation solutions
| Experimental condition | Type of solution | Amount | Recommended products |
|---|---|---|---|
| For samples containing large tissues that need to be dissociated (1-3 cerebral hemispheres from mouse or rat embryos) |
Enzyme solution | 5.0mL | Neuron Dissociation Solutions (Code: 291-78001) |
| Dispersion solution | 3.0mL | ||
| Isolation solution | 5.0mL | ||
| For samples containing small tissues that need to be dissociated (1-8 hippocampi from mouse or rat embryos) |
Enzyme solution | 2.5mL | Neuron Dissociation Solutions S (Code: 297-78101) |
| Dispersion solution | 2.5mL | ||
| Isolation solution | 2.5mL |
Dissociation and Isolation of Frozen Neurons
Frozen neurons from our company were thawed, and were dissociated and isolated in the neuron dissociation solutions.
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Product Name ① Hippocampus, from mouse (embryonic day 16) Total(cells/vial) 4.32 x 105 Viable(cells/vial) 3.83 x 105 Cell viability(%) 89 ② Cerebral cortex, from mouse (embryonic day 15) Total(cells/vial) 6.64 x 106 Viable(cells/vial) 6.09 x 106 Cell viability(%) 92 ③ Hippocampus, from rat (embryonic day 19) Total(cells/vial) 1.37 x 106 Viable(cells/vial) 1.24 x 106 Cell viability(%) 90 ④ Cerebral cortex, from rat (embryonic day 17) Total(cells/vial) 1.32 x 107 Vialble(cells/vial) 1.17 x 107 Cell viability(%) 89 ⑤ Cerebral striatum, from rat (embryonic day 17) Total(cells/vial) 2.73 x 106 Viable(cells/vial) 2.58 x 106 Cell viability(%) 95
▶The data demonstrated that the use of neuron dissociation solutions enables isolation of cells from frozen samples with high viability (~90%).
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Culture medium
Frozen nerve cells
Cryopreservation solution
Basal Medium / supplement
Related Information
Category
- Cell Culture
- Nerve Cell Culture
- Cell Dispersion / Detachment
- Life Science
- Neuroscience
- Neural Cell Culture
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