分类：Wako

Wako和光纯药CultureSure® 10w/v% Polyoxyethylene Polyoxypropylene Glycol Solution

Surfactant for insect cell culture media

CultureSure® 10w/v% Polyoxyethylene Polyoxypropylene Glycol Solution

This product is a 10% aqueous solution of a nonionic surfactant designed for cell culture applications.
In insect cell cultures, it is used as a media additive to both suppress cell aggregation and prevent cell attachment to flasks.
While acting as an antifoaming agent, this surfactant limits the interaction of cells with air bubbles, thus protecting cells against injury. It also defends cells from shear forces produced by sparging air. The beneficial effects of this surfactant have been shown to promote cell proliferation.

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Product Information

  • Liquid format
  • Endotoxin: ≦0.5 EU/mL
  • Sterility tested
  • Mycoplasma tested

References

  1. David W. M. and Charles F. G.: BIO/TECHNOLOGY, 6, 1411(1988).
  2. Jordan M. et al: Biotechnol Bioeng. , 43, 446(1994).
  3. Gilbert R. S. et al: Cytotechnology, 22, 211(1996).

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Liquid culture medium for insect culture

Medium additive reagent

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Category

  1. Cell Culture
  2. Animal Cell Culture
  3. Cell Preservation

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Wako和光纯药CultureSure® DMSO

CultureSure® DMSO

FUJIFILM Wako’s dimethyl sulfoxide (DMSO) is tested for both sterility and endotoxin content. It is filter-sterilized and thus safe to use for cell culture applications.

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Features

  • DMSO produced in Japan was distilled and then filter-sterilized with a 0.1 μm pore-size membrane.
  • High purity (≧99.9%)
  • Endotoxin- and sterility-tested.
  • Packaged in airtight glass vials with Teflon stoppers. Hermetically sealed with aluminum film.

Product Specification

Requirement Specification
Specific Gravity 1.095-1.101
Water ≦ 0.2%
Absorbance(275nm) ≦ 0.20
Ratio of Absorbance(A285/A275) ≦ 0.65
Ratio of Absorbance(A295/A275) ≦ 0.45
Residue after Evaporation ≦ 0.01%
Acidity To pass test
Analogous substances ≦ 0.1%
Assay ≧ 99.9%
Appearance Colorless Clear Liquid
Refractive Index(nD20) 1.477～1.480
Freezing Point 18℃～20℃
Bacterial endotoxins <0.25EU/mL
Sterility test To pass test

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CultureSure® cryopreservation solution

StemSure® cryopreservation solution

CultureSure® Amino Acid Series

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Category

  1. Cell Culture
  2. Animal Cell Culture
  3. Cell Preservation

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Wako和光纯药BAMBANKER™ & BAMBANKER™ Direct

Serum-Free Cell Freezing Medium

BAMBANKER™ & BAMBANKER™ Direct

BAMBANKER&trade; & BAMBANKER&trade; Direct

BAMBANKER™ is a serum-free medium for long-term freezing at -80°C and preservation of valuable culture cells such as tumor cells and normal cells.
BAMBANKER™ Direct is improved operability. It doesn’t require centrifugation for cell collecting.

User report 1 by: Shota Moriya, Department of Biochemistry, Tokyo Medical University
User report 2 by:Dr. Atsunori Sato of Tagawa Laboratory, School and Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology

  • User Report 1Jul. 01, 2017
  • User Report 2Feb. 01, 2019

  • Features
  • Operating Procedure
  • Sterility test
  • Application
  • Comparison
  • Precautions
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Features

  • Ready-to-use medium for preservation of cells
  • Usable without diluting
  • NO program freezer is required
  • Rapid and long-term freezing and preservation in a deep freezer (-80°C)
  • Serum-free

Operating Procedure

BAMBANKER™

BAMBANKER&trade; & BAMBANKER&trade; Direct

　

BAMBANKER™ Direct

BAMBANKER&trade; & BAMBANKER&trade; Direct

Sterility test

Endotoxin:　 　Chromogenic substrate method
Mycoplasma:　 　Fluorescent antibody method
Fungi and bacteria:　 　As per Japanese Pharmacopoeia
**: Certificate of analysis will be issued upon request.

Application

Cell lines

•201B7 (human iPS cell line) •3T3-L1 (mouse preadipocyte cell line) •A431 (human squamous cell carcinoma cell line)
•BAEC (bovine aortic vascular endothelial cell line) •Balb/3T3 (mouse fibroblast cell line) •C2C12 (mouse skeletal muscle cell line)
•Daudi (human B cell line) •ECV 304 (human umbilical vein endothelial cell line) •H295R (adrenal cortex cells)
•HEK 293 (human embryonic kidney cell line) •HEK293T (human fetal kidney cell line) •HeLa (human cervical cancer cell line)
•HeLa S3 (human cervical cancer cell line) •HepG2 (human liver cancer cell line) •HFF (human normal fibroblast cell line)
•Huh7 (human liver cancer cell line) •Jurkat (human leukemia T cell line) •K562 (human chronic myelogenous leukemia cell line)
•KATO III (human gastric cancer epithelial cell line) •KLM-1 (human pancreatic cancer cell line) •MDCK (canine kidney tubular epithelial cell line)
•DT40 (derived from chicken B cells) •NIH3T3 (mouse fetal skin cell line) •OKT4 (mouse hybridoma)
•OP9 (mouse myeloid stromal cells) •P3U1 (mouse myeloma cell line) •PANC-1 (human pancreatic cancer cell line)
•PC12 (rat-derived adrenal pheochromocytoma cell line) •RPE (human membrane epithelial cell line) •SNL (mouse fetal fibroblasts)
•TSU-Pr1 (human prostate cancer cell line) •Vero (African green monkey kidney cell line) •Mouse ES cell line
•Human immortalized myocytes •Human immortalized myocytes

Primary cells

•MEF (mouse fetal fibroblasts) •Human γδ T cells •Human peripheral blood-derived activated lymphocytes
•PBMC (peripheral blood mononuclear cells) •Mouse spleen-derived activated lymphocytes

Comparison

Comparison of preservation efficiency of mouse fibroblast strain

BAMBANKER™ Self prepared solution
Conditions
Cells used: Mouse fibroblast cell line
Number of cells stored: 4 × 106 cells / vial. Each cell was suspended in 1mL of preservation solution.
Storage temperature: -80°C. Cells were directly placed in storage box and cooled without using BICELL, etc.
Storage period: 45 days
Thawing method: Cells were thawed according to the conventional method.
Method of measuring
preservatiion efficiency:		 After thawing, 5 x 104 cells were inoculated into a 24-well culture plate with 1 mL DMEM medium, and the cell density per culturing area was measured by successive timlapse imaging.
Data by Shota Moriya, Department of Biochemistry, Tokyo Medical University

Better cell viability was obtained using BAMBANKER™ than self-prepared preservative solution.

　

　

Comparison of Bambanker™ with other relevant products

[Cryopreservation Test]

Result

*1: Storage period of Cell Line at -80ºC

 Media tested
1. Bambanker™ (LYMPHOTEC Inc.)
2. Medium with serum (Company A)
3. Serum-free Medium (Company A)

BAMBANKER&trade; & BAMBANKER&trade; Direct

Precautions

  • Use only for research purposes, not for human use.
  • Prior to using this medium, perform the confirmation test on the cells under study.
  • The manufacturer shall not be responsible for any accident or damage caused by the use of this product.
  • If you have any questions on the use of this medium, please contact the local distributor.

References

  • T.Hikichi, et al; Differentiation Potential of Parthenogenetic Embryonic Stem Cells Is Improved by Nuclear Transfer, Stem Cells, 25, 46-53(2007)
  • Durruthy-Durruthy, J. et al. : Nature Genetics, 48, 44-52 (2016).
  • Sugii, S. et al. : Nature Protocols, 6, 346–358 (2011).
  • Zaidi, SK. et al. : Proc Natl Acad Sci U S A, 104, 19861–19866 (2007).

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Category

  1. Cell Culture
  2. Animal Cell Culture
  3. Cell Preservation

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Wako和光纯药CultureSure® Media Supplement Series

Amino Acids, Vitamins, Carbohydrates, and Inorganic Salts

CultureSure® Media Supplement Series

FUJIFILM Wako offers the CultureSure® line of reagents that have been specifically formulated and validated for use in cell culture applications. They are ideal for clinical research and product development in the fields of regenerative medicine and biopharmaceutical engineering.
CultureSure® media supplements (amino acids, vitamins, carbohydrates, and inorganic salts) are free of animal-derived components. These reagents are also tested for endotoxin levels and the contamination of microorganisms (including mycoplasma species). Moreover, they are produced by manufacturers with effective change management practices. These stringent standards in quality control ensure the safety and reliability of this media supplement series.

  • What Are CultureSure® Media Supplements?
  • Managing a Seamless Transition to Mass Production
  • Aichi M-1 Plant: A Facility Dedicated to Culture Media Manufacturing
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What Are CultureSure® Media Supplements?

  • Animal Component Free
    FUJIFILM Wako defines animal component-free products as those manufactured from materials that do not contain any animal-derived ingredients at either the primary or secondary level.
  • Cell Culture Tested
    These media supplements are endotoxin-free. They are also tested for mycoplasma and other microbial contamination.
  • Class 100,000 Certified
    Our reagents are manufactured in a certified clean-room environment.

Managing a Seamless Transition to Mass Production

  • Implement an effective change management program.
  • Supply CultureSure® media supplements in bulk quantities.
  • Achieve and maintain the high levels of quality required for product commercialization.※
    ※ As CultureSure® products are used as reagents, they are manufactured in accordance with the ISO standards. Upon request, they can be produced under the control of our in-house standards that generally comply with the requirements of GMP.

Supplementary Information: Examples of Regulatory Requirements for Different Grades of Biomedical Products

Regulatory
Requirement		 Reagent
Grade		 CultureSure® Japanese
Pharmacopoeia
Products
Manufacturing Environment Uncontrolled Cleanroom Cleanroom
Change Management Inapplicable Applicable Applicable
Document Management ISO ISO GMP
Product Testing Japanese Industrial Standards Contamination of endotoxins
and microorganisms
(including mycoplasmas)		 Japanese Industrial Standards

Aichi M-1 Plant: A Facility Dedicated to Culture Media Manufacturing

This factory was established to produce culture media and reagents for the commercialization of biologics and regenerative medicine products.
[FUJIFILM Wako’s GMP-class manufacturing facility]

CultureSure® Media Supplement Series
  • Carefully managed and operated for culture media production, using the ICH Q7 guideline (GMP Guide for Active Pharmaceutical Ingredients) as a reference
  • Completely validated in accordance with regulatory requirements
    ranging from facility and utility maintenance to manufacturing, testing, and cleaning processes
  • Fully guaranteed to meet stringent quality requirements.
    We ensure that our raw powder materials for culture media are sourced only from reputable suppliers who maintain appropriate manufacturing practices.

FUJIFILM Wako can also produce customized media. For more information, contact our sales representatives in your area.

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Amino acids

Sugar

Vitamins

Inorganic salts

Other

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Category

  1. Cell Culture
  2. Animal Cell Culture
  3. Medium Additive

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Wako和光纯药Cytokines (Bulk Order)

Cytokines (Bulk Order)

Our cytokines are available in large quantities. Please contact the distributors for the pricing of bulk orders.

What are animal-derived-free cytokines?
Animal-derived-free cytokines are expressed and purified from E. coli that was cultured without any animal-derived materials. There is only a minimal risk of viral contaminations that would otherwise be caused by the presence of animal- derived raw materials. These cytokines can be used in the same way as regular cytokines.

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Human, recombinant (A-N)

Human, recombinant (O-V)

Mouse, recombinant

Rat, recombinant

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To prepare cytokines

Medium / Medium additives etc.

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Category

  1. Cell Culture
  2. Animal Cell Culture
  3. Cytokine / Growth Factor

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